CRISPR Base Editors Are Often Off Base with RNA

April 19, 2019

(GEN) – “Most investigation of off-target base editing has focused on DNA, but we have found that this technology can induce large numbers of RNA alterations as well,” said Joung. “This surprising finding suggests the need to look at more than just genetic alterations when considering unintended off-target effects of base editors in cells. We also show the feasibility of reducing these effects by creating variants that selectively reduce off-target RNA editing while preserving the intended on-target DNA activity.”

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